A vast neural tracing effort by a team of Janelia scientists has upped the number of fully-traced neurons in the mouse brain by a factor of 10. Researchers can now download and browse the data in three dimensions.
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Hemidriver Drosophila Lines ("Split-halves")
These split-GAL4 driver Drosophila lines allow the generation of cell-type specific gene expression. They are freely available through the Bloomington Stockcenter through a large coordinated effort from Janelia Drosophila Resources and the National Institutes of Health.
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Janelia Fluor Dyes
These bright and photostable fluorescent labels enable imaging and tracking of individual molecules inside living cells.
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GCaMP6
The GCaMP6 family of GECIs is a collection of green fluorescent indicator proteins that facilitate the measurement of synaptic calcium signals, enabling reliable detection of neuronal responses in vivo. The next-generation GCaMP6s, GCaMP6m, and GCaMP6f sensors offer increased sensitivity (single action potential detection in vivo) and improved kinetics.
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CaMPARI
This photoconvertible protein enables imaging of the calcium activity history of large areas and populations of cells. It is based on EosFP, a fluorescent protein that shows emission changes from green to red upon irradiation with UV light. It is not restricted to the field of view of a microscope, as during real-time calcium imaging.
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Lattice Light Sheet Microscope
This microscope uses a Bessel beam to illuminate a sample with light sheets that are sufficiently thin to achieve isotropic resolution high-speed 3D fluorescent imaging. The technology can visualize cellular processes in spatiotemporal detail not attainable with other fluorescent microscopy techniques.
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